EFFECT OF GENISTEIN ON EXPRESSION OF MONOCYTE CHEMOTACTIC PROTEIN-1 INDUCED BY OXIDIZED LIPOPROTEINS IN HUMAN UMBILICAL SMOOTH MUSCLE CELLS / 营养学报
Acta Nutrimenta Sinica
; (6)1956.
Article
en Zh
| WPRIM
| ID: wpr-555335
Biblioteca responsable:
WPRO
ABSTRACT
Objective: To study the effect of genistein(Gen) on expression of monocyte chemotactic protein-1 ( MCP-1) mRNA and MCP-1 induced by oxidized low density lipoprotein (ox-LDL) in human umbilical venous smooth mulscle cells (hUVSMC). Methods: Gen at different doses (1.0?10-5、3.0?10-5、9.0?10-5mol/L) were used to observe the effect on expression of MCP-1 mRNA and MCP-1 induced by ox-LDL in hUVSMC cultures and compared with the effect of 17b-estradiol(17b-E). RT-PCR and ELISA were used to measure expression of MCP-1 mRNA and MCP-1 respectively. Results: Gen significantly inhibited the expression of MCP-1 mRNA and MCP-1 in cell culture supernant . There was no significant difference in the inhibitory effect between high concentration of Gen and physiological level of 17b-E . Conclusion: Gen can down-regulate hUVSMC expression of MCP-1 mRNA and MCP-1. It suggests that this may be the important antiatherogenic mechanism of Gen.
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1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Nutrimenta Sinica
Año:
1956
Tipo del documento:
Article