Construction of soluble anti-digoxin ScFv antibodies and diabody / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1985.
Article
en Zh
| WPRIM
| ID: wpr-547219
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To clone soluble human anti-digoxin antibodies from large phage antibody library and construct a vector that expresses diabody.Methods:Soluble ScFvs were prepared through infecting E coli.HB2151 with the selected phage antibodies and induced with IPTG.The diabody vector was modified by enzyme digestion and checked by SDS-electrophoretogram.Results:It was showed by ELISA that soluble ScFv had specific binding ability to digoxin.The vector to express a soluble diabody was obtained by genetic modification,which was shown by Western blot.Conclusion:Soluble human anti-digoxin antibodies were successfully obtained from phage antibodies.The vector is efficient in creating diabody.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Año:
1985
Tipo del documento:
Article