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Preliminary study of aspisol inhibition on proliferation of breast cancer cells / 中国癌症杂志
China Oncology ; (12)2006.
Article en Zh | WPRIM | ID: wpr-545490
Biblioteca responsable: WPRO
ABSTRACT
Background and purpose:Many studies indicate that nonsteroidal anti-inflammatory drugs(NSAIDs) may inhibit cancer growth.However,the molecular mechanisms may involve different pathway and still remain unclear.The aim of this experiment was to investigate the influence of aspisol against breast cancer lines,including MDA-MB-231(estrogen receptor-negative),MCF-7(estrogen receptor-positive),and reveal the potential signaling pathway mechanism of aspisol effect on breast cancer lines.Methods:MDA-MB-231 and MCF-7 human breast cancer cell lines were treated with aspisol in vitro.Cell proliferation was evaluated by MTT assay and rate of apoptosis were determined by flow cytometry.Extracellular signal regulated kinase(ERK),phosphor-ERK(P-ERK) protein expressed in breast cancer cell lines were analyzed by Western blot.Results:①The results of MTT assay demonstrated that the growth of MDA-MB-231,MCF-7 cells were inhibited by aspisol in a time-and dose-dependent fashion(P0.05).Conclusions:aspisol inhibits proliferation and induces apoptosis not only in ER-positive but also in ER-negative breast cancer cells.The mechanism may relate to ERK signal pathway.
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Texto completo: 1 Base de datos: WPRIM Idioma: Zh Revista: China Oncology Año: 2006 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Idioma: Zh Revista: China Oncology Año: 2006 Tipo del documento: Article