Preparation of monoclonal antibodies against ?-amyloid peptide_(1-42) / 西安交通大学学报(医学版)
Article
en Zh
| WPRIM
| ID: wpr-540304
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WPRO
ABSTRACT
Objective To prepare a hybridoma secreting stab le monoclonal antibodies against ?-amyloid peptide (A? 1-42) with high titer. Methods By genetic engineering technology, A ? gene was recombined with the MIR of HBcAg to get the A? and HBcAg fusi on protein. Spleen cells from BALB/c mice immunized with A? and HBcAg f usion protein were fused with mouse myeloma cells SP2/0. Results Two strains of hybridomas (1H 7 and 1F 3) secreting stable monoclonal antibodies raised against A? 1-42 were ob tained. The subtypes of A? 1-42 antibodies were IgG 3. C onclusion The A? 1-42 monoclonal antibodies obtained have high titers and specificity.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Xi'an Jiaotong University(Medical Sciences)
Año:
2004
Tipo del documento:
Article