Effects of salvia miltiorrhizae on L-Ca current in ventricular myocyte of guinea pig during normoxia, acute hypoxia and reoxygenation / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
en Zh
| WPRIM
| ID: wpr-524074
Biblioteca responsable:
WPRO
ABSTRACT
AIM: To observe the changes of Ca-L current (ICa-L) and to investigate the mechanism of salvia miltiorrhizae (SM) for eliminating Ca~2+ overloaded in cells during acute hypoxia/reoxygenation. METHODS: The whole cell patch clamp technique was applied to study the changes of ICa-L. Different concentrations (32, 320, ~3 200 mg/L) of SM were added to the ventricular myocytes isolated from guinea pigs by enzyme digestion. RESULTS: SM (32, 320, ~3 200 mg/L) decreased the amplitude of ICa-L in a concentration-dependent manner regardless of these cells were under normoxia, hypoxia or reoxygenation. Furthermore, SM at low concentration (32 mg/L) was more effective to hypoxia or reoxygenation-treated cells than that to the cells under normoxia condition. CONCLUSION: These results indicate that SM effectively decreases the abnormal raised amplitude of ICa-L in ventricular myocytes under hypoxia or reoxygenation conditions, preventing Ca~2+ overloaded in the cells. [
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Año:
1986
Tipo del documento:
Article