Primary study on the differentiation mechanism of embryonic stem cells to epidermal like cells induced by human amnion / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
en Zh
| WPRIM
| ID: wpr-522984
Biblioteca responsable:
WPRO
ABSTRACT
AIM: To investigate the mechanism by which human amnion induced mouse embryonic stem (ES) cells to differentiate into epidermal like cells. METHODS: ES-BALB/c cells were cocultured with human amnion in transwells for 4-5 days, and those cultured alone without amnion were taken as control group. The morphological differentiation were observed. The committed differentiation of ES cells into epidermal like cells were detected by integrin-?_1, CK19, CK15 and involucrin immunohistochemistry, respectively. RESULTS: After 4-5 days of coculture, ES cells differentiated into single layer of epidermal like cells, fitted tightly, with polyhedral in shape. The immunohistochemical staining results showed that, most of the cells were integin-?_1 positive, only a few cells were CK19 and CK15 positively stained. Most of the cells in control group died, the survived ones were different in morphological shapes, and no integrin-?_1, CK19 and CK15 positive cells were found. CONCLUSION: Soluble substances secreted by human amnion may play an important role in inducing the differentiation of mouse ES cells into epidermal like cells. [
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Año:
2000
Tipo del documento:
Article