Detection of xylazine and 2,6-xylidine in blood and urine by SPE-HPLC/MS/MS / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6): 182-185, 2017.
Article
en Zh
| WPRIM
| ID: wpr-512031
Biblioteca responsable:
WPRO
ABSTRACT
Objective To develop an HPLC-MS/MS method for simultaneous determination of xylazine and 2,6-xylidine in body fluid samples.Methods The samples were extracted by HLB SPE,separated by Waters Atlantis dC18 chromatographiccolumn,and then detected in the MRM scan ion mode under positive ionization condition.Results The recoveryrates of this method were between 70.5％ and 79.8％ with the range of RSD rfrom8.2％ to 10.5％.The limits of detection ofxylazine and 2,6-xylidine in blood and urine samples were 0.4 and 0.3 ng/mL,and the limits of quantitation were 1.2 and 1.0 ng/mL,respectively.Conclusion This method is of high sensitivity,good specificity and good reproducibility,and thus could besuitable for accurate quantification of xylazine in blood and urine samples.
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Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Forensic Medicine
Año:
2017
Tipo del documento:
Article