Effects of estrogen on osteogenesis and fibroblast-related gene expression of bone marrow mesenchymal stem cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
; (53): 4869-4875, 2016.
Article
en Zh
| WPRIM
| ID: wpr-498269
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND:Bone marrow mesenchymal stem cel s have been shown to be differentiated into periodontal ligament fibroblasts when co-cultured with periodontal ligament cel s. Existing studies have shown that estrogen has the ability to influence bone marrow regeneration. OBJECTIVE:To investigate the effects of estrogen on osteogenesis and fibroblast-related factors alkaline phosphatase, type I and III col agen in bone marrow mesenchymal stem cel s. METHODS:Bone marrow mesenchymal stem cel s isolated from Beagle dogs were treated with estrogen. Osteogenesis and fibroblast-related mRNA and protein expression of bone marrow mesenchymal stem cel s was determined by RT-PCR and western blot assay, respectively. RESULTS AND CONCLUSION:mRNA and protein expression of type I and III col agen in bone marrow mesenchymal stem cel s was upregulated fol owing estrogen treatment;especial y, in contrast with type III col agen, the changes of type I col agen were more obvious. Estrogen did not influence mRNA and protein expression of alkaline phosphatase. These findings suggest that estrogen promotes the differentiation of bone marrow mesenchymal stem cel s into fibroblasts, whereas does not impact the genes involved in parodontium mineralization.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2016
Tipo del documento:
Article