Optimization and application of VSVG/HIV-1 NL4-3 Luc system for screening of anti-HIV-1 compounds / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 433-438, 2016.
Article
en Zh
| WPRIM
| ID: wpr-487670
Biblioteca responsable:
WPRO
ABSTRACT
Aim To establish and optimize the VSVG/HIV-1NL4-3 Luc pseudovirus model for anti-HIV drugs screening. Methods The infectivity of VSVG/HIV-1 NL4-3 Luc in 4 different cell lines was investigated according to the method of the lucifer-ase activity analysis system of Promega company. 3 different ex-perimental settings were used to detect the activities of approved anti-HIV drugs to confirm the feasibility and effectiveness of the system. Finally, some potential compounds were screened for their anti-HIV activities, and their antiviral activities against the pseudovirus were compared with HIV-1ⅢB . Results The pseud-ovirus showed the strongest replication ability in CRFK cells, and a clear dose-effect relationship was found between the report gene expression level and the virus quantity. Comparing the EC50 of different positive inhibitors against VSVG/HIV-1 NL4-3 Luc on 3 kinds of experimental conditions, 3rd scheme is the best. Finally, the system was used to screen compounds, the EC50 s a-gainst pseudovirus were similar to those in HIV-1ⅢB . Conclusion An optimized VSVG/HIV-1 NL4-3 Luc anti-HIV screening sys-tem has been successfully developed.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Año:
2016
Tipo del documento:
Article