Effect of miR-199a/b-3p on cell motility of breast cancer cells / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1242-1244, 2015.
Article
en Zh
| WPRIM
| ID: wpr-476755
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To analyze the inhibiting mechanism of microRNA-199a/b-3p ( miR-199a/b-3p) on cell motility of breast cancer cells.Methods:The expression of PAK4 in MDA-MB-231 cells transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of MDA-MB-231 cells transfected with miR-199a/b-3p or PAK4 SiRNA were analysed by cell migration assay,invasion assay and protrusion dynamics.Results: MiR-199a/b-3p could suppress the expression of PAK 4 in MDA-MB-231 cells.Comparing with normal control ,miR-199a/b-3p or PAK4 SiRNA could suppress the migration ,invasion and membrane protrusion of MDA-MB-231 cells.Conclusion:miR-199a/b-3p could suppress the expression of PAK4,which are considered key breast cancer suppressors and inhibit the cell motility of breast cancer cells.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Año:
2015
Tipo del documento:
Article