Objective:To observe the effects of pretreatment and treatment with electroacupuncture on the abilitv of learning and memory in rats with ischemia-reperfusion injury,and to provide the research basis for preventing and treating the disorder of leaming and memory in patients with apoplectic sequelae.Methods:Seventy-two SD rats,clean class,were randomly divided into sham operation group,model group,pretreatment group,and treatment group.The step-through latency and number of errors,nerve injury score,and the number of cholinoceotor in the affected hippocampus were recorded to analyze the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion iniury.Results:1)The rats with ischemia had various signs of nerve injury.Compared with that in the sham operation rats,the nerve injury score in the model rats was lower,the step-through latency and number of errors were increased,and the content of special mAchR was decreased(P＜0.05):2)Electroacupuncture could decrease the nerve injury score of the model rats.Compared with those in the model group,the step-through latency and number of errors all had significant difierence in the pretreatment and treatment groups(P＜0.05);3)Compared with that in the model group.the content of mAchR was increased in the pretreatment group(P＜0.05),and increased a little in the treatment group.Conclusion:Pretreatment and treatment with electroacupuncture can prolong step-through latency,and induce number of errors.Electroacupuncture pretreatment can increase the content of mAchR induced by ischemia-reperfusion injury,to improve the ability of learning and memory.Electroacupuncture pretreatment plays an important role in preventing the disorder of learning and memory in patients with ischemic cerebrovascular disease.