Amplification of cytochrome C oxidase subunit Ⅰ gene of Brandt's Vole by nested PCR / 中华地方病学杂志
Chinese Journal of Endemiology
; (12): 70-72, 2015.
Article
en Zh
| WPRIM
| ID: wpr-470341
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WPRO
ABSTRACT
Objective To determine a method for amplification of cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene of Brandt's vole.Methods 发he Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia,and DNA was extracted from liver tissue.CO Ⅰ gene was amplified by nested PCR and sequenced afterwards.Results A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers,respectively,which were consistent with expected sizes.A total of 12 segments of Brandt's Vole CO Ⅰ gene sequences were amplified by PCR and verified by sequencing.一he sequence number was KF182196-KF182207 in GenBank.After gene sequence alignment of the 12 CO Ⅰ gene sequences,it was found that the similarity was 100％,and no base mutation.Conclusion CO Ⅰ gene of Brandt's Vole could be amplified by nested PCR without pseudo gene.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Endemiology
Año:
2015
Tipo del documento:
Article