Methodological Research on TLC Identification of Radix Mirabilis himalaica / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
; (12): 2725-2730, 2014.
Article
en Zh
| WPRIM
| ID: wpr-461641
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to establish the TLC identification method of Radix Mirab ilis himalaic a. The β-sitosterol and daucosterol were used as the reference substances. The single-factor test was used. A variety of factors which affected TLC were systematically investigated to filter out the best TLC conditions for identification of different batches of medicines. The results showed that the best TLC conditions were as follows: silica gel G plates, extraction solvent (methanol), reagent (5% sulfuric acid in ethanol), extraction method (ultrasonic extraction with methanol), ex-tracted time (30 min), the agent (petroleum ether-ethyl acetate-acetone (5:2:1)) and sample volume (6 μL). It was concluded that the method, which had high separation degree, was reproducible and simple. It can be used as the quality control of Radix Mirab ilis himalaic a.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
World Science and Technology-Modernization of Traditional Chinese Medicine
Año:
2014
Tipo del documento:
Article