PCR sequencing for detection and gene typing of hantavirus isolated from rodents / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
; (6): 44-49, 2014.
Article
en Zh
| WPRIM
| ID: wpr-452707
Biblioteca responsable:
WPRO
ABSTRACT
Objective To evaluate the application value of PCR-sequencing in clinical detection of hantavirus in rodents .Methods Based on 7 subtypes and 24 strains of representative hantavirus strains downloaded from Genbank , the virus S gene fragments were used for primer design and neighbor joining method was applied for phylogenetic analysis . Thereafter, we identified hantavirus strains isolated from wild rodents in recent years in Zhejiang Province by this method . Results The 24 analyzed strains were divided into 5 regions in the phylogenetic tree .Four of them with topology structure were more stable .Eleven strains of the virus were amplified by PCR and sequenced , and the results showed that the prim-ers were with high sensitivity and specificity .Three HTN strains and 1 strain of serotype SEO were distinguished from 9 strains of unknown strains isolated in Zhejiang Province .We also found that 5 strains of hantavirus belonging to two un-known serotypes .Discussion Our results suggest that the PCR-sequencing method proposed in this study can be used for clinical detection of hantavirus .
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Laboratorium Animalis Scientia Sinica
Año:
2014
Tipo del documento:
Article