Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method / 药学学报
Acta Pharmaceutica Sinica
; (12): 244-8, 2014.
Article
en Zh
| WPRIM
| ID: wpr-448727
Biblioteca responsable:
WPRO
ABSTRACT
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2014
Tipo del documento:
Article