Isolation,culture, adipocgenic and osteogenic induction of Tupaia bone marrow mesenchymal stem cells / 中国比较医学杂志
Chinese Journal of Comparative Medicine
; (6): 10-13, 2014.
Article
en Zh
| WPRIM
| ID: wpr-446196
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the isolation,culture, adipogenic and osteogenic induction Tupaia bone marrow mesenchymal stem cells(BM-MSCs).Method The BM-MSCs from tupaia were isolated and expended by combination of gradient centrifugation and adherence culture , then subcultured and observed for morphology under inverted phase contrast microscope.BM-MSCs were induced to adipocytes .and osteoblasts in vitro Result Cells were spindle or triangle-shaped, and clone proliferation .Cells were successfully induced into adipocytes .and osteoblasts Conclusions The method of isolation BM-MSCs from tupaia by combination of gradient centrifugation and adherence culture is simple and feasible , BM-MSCs have differentiation potential into adipocytes and osteoblasts .
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Comparative Medicine
Año:
2014
Tipo del documento:
Article