Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System / 中国病毒学·英文版
Virologica Sinica
; (6): 338-346, 2011.
Article
en Zh
| WPRIM
| ID: wpr-423816
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WPRO
ABSTRACT
Crimean-Congo Haemorrhagic Fever Virus(CCHFV)is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes,high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research,the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope,Virus-Like Particles (VLPs)with various size and morphology were observed in cytoplasmic vesicles in the infected cells.Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy(IEM).
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WPRIM
Idioma:
Zh
Revista:
Virologica Sinica
Año:
2011
Tipo del documento:
Article