A modified isolation method for mouse intestinal intraepithelial lymphocytes / 肠外与肠内营养
Parenteral & Enteral Nutrition
; (6): 106-108, 2010.
Article
en Zh
| WPRIM
| ID: wpr-415291
Biblioteca responsable:
WPRO
ABSTRACT
Objective: To explore and modify the isolation method for mouse intestinal intraepithelial lymphocytes. Methods: Epithelium mucosae of mouse small intestine was incubated in iced bath and shaked in PBS containing DTT. The cell suspension was obtained after filtration with 80 and 400- screen mesh trap valve in turn. The yield, viability and purity of intestinal intraepithelial lymphocytes were observed to estimate the feasibility. Results: About (5.6±0.7)×10～6 intestinal intraepithelial lymphocytes were obtained from every 20cm samll intestine. The viabilty of intestinal intraepithelial lymphocytes was (90.46±5.71)% and the purity was (92.21±5.20)%. Conclusion: Compared with other reported isolation methods, the modifled method is convenient and esay to handling.The yield, viability and purity are high enough to be used for intestinal intraepithelial lymphocytes studies.
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1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Parenteral & Enteral Nutrition
Año:
2010
Tipo del documento:
Article