Effect of docetaxel on expression of eIF3a in human lung cancer A549 cell line / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban
; (12): 771-776, 2010.
Article
en Zh
| WPRIM
| ID: wpr-402305
Biblioteca responsable:
WPRO
ABSTRACT
Objective To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A (eIF3a) in lung cancer cell line. Methods The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time. Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and α-tubulin, respectively. Results Docetaxel did not affect α-tubulin expression at either mRNA level or protein level. When A549 cells were treated with high concentration of docetaxel (30 μg/L), the expression level of eIF3a mRNA tended to increase in a time-dependent manner. Protein expression level of α-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.Conclusion Docetaxel could slightly increase the expression of eIF3a mRNA, and eIF3a does not regulate the expression of α-tubulin in A549 cells treated by docetaxel.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Zhongnan Daxue xuebao. Yixue ban
Año:
2010
Tipo del documento:
Article