Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation / 亚洲男科学杂志(英文版)
Asian j. androl
; Asian j. androl;(6): 227-235, 2008.
Article
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| WPRIM
| ID: wpr-359958
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate whether early apoptotic changes in spermatozoa can be significant markers for sperm quality.</p><p><b>METHODS</b>Two early apoptotic changes in the semen of 56 men were assessed using Annexin V (AN)/propidium iodide (PI) staining for phosphatidylserine externalization and JC-1 staining for mitochondrial membrane potential (MMP). The results were compared with conventional semen parameters and DNA fragmentation identified using the TUNEL assay.</p><p><b>RESULTS</b>The different labeling patterns in the bivariate Annexin V/PI analysis identified four distinctive spermatozoa populations. The percentage of AN(-)/PI(-) spermatozoa positively correlated with conventional semen parameters and MMP, but negatively correlated with TUNEL (+) spermatozoa. As for the AN(-)/PI(+) fraction, we found an opposite result in comparison to AN(-)/PI(-) spermatozoa. The level of early apoptotic AN(+)/PI(+) spermatozoa negatively correlated with MMP and sperm motility. The level of late apoptotic AN+/PI+ spermatozoa negatively correlated with conventional semen parameters and MMP, and positively correlated with TUNEL (+) spermatozoa. MMP positively correlated with conventional semen parameters, but negatively correlated with TUNEL (+) spermatozoa.</p><p><b>CONCLUSION</b>Although early apoptotic AN+/PI(-) spermatozoa only negatively correlates with sperm motility, the differences in proportion of each subpopulation of spermatozoa (especially, the percentage of AN(-)/PI(-) spermatozoa), and decreased MMP might be significant markers for diagnosing male infertility. They possibly bring additional information to predict the outcome of in vitro fertilization.</p>
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Base de datos:
WPRIM
Asunto principal:
Fisiología
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Semen
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Espermatozoides
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ADN
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Apoptosis
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Biología Celular
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Diagnóstico
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Fragmentación del ADN
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Potencial de la Membrana Mitocondrial
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Infertilidad Masculina
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Asian j. androl
Año:
2008
Tipo del documento:
Article