Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector / 浙江大学学报（英文版）（B辑：生物医学和生物技术）
Journal of Zhejiang University. Science. B
; (12): 728-733, 2008.
Article
en En
| WPRIM
| ID: wpr-359378
Biblioteca responsable:
WPRO
ABSTRACT
A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008-0.160 microg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Espectrofotometría Ultravioleta
/
Medicamentos Herbarios Chinos
/
Química
/
Cromatografía Líquida de Alta Presión
/
Salvia miltiorrhiza
/
Evaluación Preclínica de Medicamentos
/
Hidroxibenzoatos
/
Métodos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Año:
2008
Tipo del documento:
Article