The effect of advanced glycosylation end products on the expression of fibronectin and the regulation of protein kinase C in human peritoneal mesothelial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 365-372, 2012.
Article
en Zh
| WPRIM
| ID: wpr-358740
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of advanced glycosylation end products (AGEs) on the production of fibronectin (FN) in human peritoneal mesothelial cells (HPMC) in vitro and the role of protein kinase C (PKC) in this course.</p><p><b>METHODS</b>The AGE-human serum albumin (HSA) (0, 100, 500, 1 000 microg/ml) was used in culture medium to stimulate the HPMC. The mRNA level of FN was measured with real-time PCR, moreover, the protein level of FN in HPMC was detected by ELISA. With the method of ELISA, the PKC activities were observed. Inhibitors or activators of PKC were used to observe the roles of PKC pathways on the AGE-HSA stimulated productions of FN in HPMC.</p><p><b>RESULTS</b>AGE-HSA activated PKC in HPMC in a dose, time-dependent manner (P < 0.05). AGE-HSA up-regulated the expression of FN mRAN and protein in dose- and time-dependently (P < 0.01); PKC activator phorbol 12-myristate 13-acetate (PMA) induced FN expression, respectively depletion of PKC and calphostin C, a PKC inhibitor, effectively prevented both PMA and AGE-HSA-induced expression of the FN (P < 0.05).</p><p><b>CONCLUSION</b>AGEs can increase the activities of PKC. AGEs can directly increase FN expression in HPMC which may contribute to peritoneal fibrosis and this is regulated by PKC.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Peritoneo
/
Farmacología
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Proteína Quinasa C
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Células Cultivadas
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Fibronectinas
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Productos Finales de Glicación Avanzada
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Secreciones Corporales
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Biología Celular
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Epitelio
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2012
Tipo del documento:
Article