Screening and identification of phage-displayed polypeptides specifically binding to human gastric cancer with high metastatic potential to peritoneum / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 397-400, 2005.
Article
en Zh
| WPRIM
| ID: wpr-358622
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>By means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.</p><p><b>METHODS</b>Two human gastric cancer cell lines were used: GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.</p><p><b>RESULTS</b>After three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides: TLNINRLILPRT and SMSI(X)SPYI(XXX).</p><p><b>CONCLUSION</b>Two peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Péptidos
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Neoplasias Peritoneales
/
Unión Proteica
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Neoplasias Gástricas
/
Sitios de Unión
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Ensayo de Inmunoadsorción Enzimática
/
Sensibilidad y Especificidad
/
Biblioteca de Péptidos
/
Análisis por Matrices de Proteínas
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2005
Tipo del documento:
Article