Cloning and sequence analysis of Chlamydia trachomatis heat shock protein 10 / 中华男科学杂志
National Journal of Andrology
; (12): 687-689, 2003.
Article
en Zh
| WPRIM
| ID: wpr-357106
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.</p><p><b>METHODS</b>cHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.</p><p><b>RESULTS</b>cHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.</p><p><b>CONCLUSIONS</b>cHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Bacterianas
/
Sangre
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Química
/
Chlamydia trachomatis
/
Reacción en Cadena de la Polimerasa
/
Clonación Molecular
/
Chaperonina 10
/
Alergia e Inmunología
Límite:
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2003
Tipo del documento:
Article