Long distance vectorette PCR for detecting translocation of IgH gene in switch regions of patients with multiple myeloma / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 460-463, 2005.
Article
en Zh
| WPRIM
| ID: wpr-356537
Biblioteca responsable:
WPRO
ABSTRACT
Chromosomal translocations involving immunoglobulin heavy chain (IgH) locus at 14q32 are commonly seen in multiple myeloma (MM), which may involve in pathogenesis and associate with prognosis. To rapidly detect the occurrence of translocation involving IgH locus and the fellow chromosomes and isolate the break points, According to the break points occured in switch regions, patents and references, a long distance vectorette PCR method was developed. The results showed that using this method, a 3.5 kb PCR product from MM cell line U266 was gained, each ends aligned with 11q and IgHalpha 1 switch region, respectively. It is concluded that LDV-PCR is effective detection technique. Detecting China MM patients by using this method can interpret some specific clinic statues and pathogenesis mechanisms, and help to find new therapy targets and to assess the patient prognosis.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Translocación Genética
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Cromosomas Humanos Par 11
/
Cromosomas Humanos Par 14
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Región de Cambio de la Inmunoglobulina
/
Reacción en Cadena de la Polimerasa
/
Cadenas Pesadas de Inmunoglobulina
/
Línea Celular Tumoral
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Article