Interaction between TGF-beta1/Smad pathway and ERK pathway in vascular smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 15-18, 2010.
Article
en Zh
| WPRIM
| ID: wpr-356229
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate if the interaction between TGF-beta1/Smad pathway and ERK pathway in vascular smooth muscle cells exists.</p><p><b>METHODS</b>The rat arota was removed. The primary VSMC were isolated and cultured in vitro, then the VSMC were divided into four groups: (1) control group, (2) (TGF-beta1 group, (3) ERK blocking agent group, (4) TGF-beta1 + ERK blocking agent group. The expression of Smad2/3, ERK1/2 proteins, the content of phosphorylated ERK1/2 and Smad2/3 proteins were detected by Western blot, and the expression of Smad2/3 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR) .</p><p><b>RESULTS</b>(1) In contrast to control group, the content of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-beta1 group was increased (P < 0.05), that in ERK blocking agent group was decreased (P < 0.05). There was no difference between control group and TGF-beta1 + ERK blocking agent group. Compared with TGF-beta1 group, the contents of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-beta1 + ERK blocking agent group was decreased (P < 0.05). There was no difference in the expression of Smad2/3 and ERK1/2 proteins among different groups. (2) There were no differences in expression of Smad2 and Smad3 mRNA among different groups.</p><p><b>CONCLUSION</b>(1) TGF-beta1 can induce Smad2/3 proteins to be phosphorylated dependent on the activated ERK pathway. (2) ERK pathway does not effect the expression of Smad2/3 at the level of protein and mRNA.</p>
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Base de datos:
WPRIM
Asunto principal:
Aorta
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Fosforilación
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Fisiología
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Transducción de Señal
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Células Cultivadas
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Ratas Wistar
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Sistema de Señalización de MAP Quinasas
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Biología Celular
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Miocitos del Músculo Liso
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Proteína Quinasa 3 Activada por Mitógenos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2010
Tipo del documento:
Article