Detection of bcr/abl fusion gene in chronic myeloid leukemia by fluorescence in situ hybridization and nested reverse transcription polymerase chain reaction / 中华血液学杂志
Chinese Journal of Hematology
; (12): 235-237, 2003.
Article
en Zh
| WPRIM
| ID: wpr-354882
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the bcr/abl fusion gene in chronic myeloid leukemia (CML) for assisting in clinical diagnosis.</p><p><b>METHODS</b>Fluorescence in situ hybridization (FISH) and nested reverse transcription polymerase chain reaction (RT-PCR) were used.</p><p><b>RESULTS</b>Out of 16 CML patients, the results of FISH was not consistent with that of nested RT-PCR in 3 cases. When compared with the results of Northern blot, it was found that nested RT-PCR was more sensitive than FISH, but might give false positive results. Moreover, FISH was the easier method for detecting rarer types of bcr/abl transcripts. For detecting the latter occasions nested RT-PCR have to design several specific primers.</p><p><b>CONCLUSION</b>It could take their advantages of applying FISH and nested RT-PCR at the same time to make the results more accurate and reliable for the diagnosis, prognosis and monitor of minimal residual disease.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Leucemia Mielógena Crónica BCR-ABL Positiva
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Proteínas de Fusión bcr-abl
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Sensibilidad y Especificidad
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Hibridación Fluorescente in Situ
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Genética
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Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Aged
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2003
Tipo del documento:
Article