A study on the transdifferentiation of adipose mesenchymal stem cells into hepatocytes / 中华肝脏病杂志
Zhonghua ganzangbing zazhi
; Zhonghua ganzangbing zazhi;(12): 601-604, 2007.
Article
en Zh
| WPRIM
| ID: wpr-354696
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possibility of transdifferentiation of adipose mesenchymal stem cells (AMSCs) into hepatocytes.</p><p><b>METHODS</b>Human omentum adipose tissue was dispersed with collagenase I. Cells collected were cultured in a DMEM-F12 medium containing 2% FBS supplemented with 20 ng/ml HGF, 10 ng/ml FGF4, 1xITS and 0.1 micromol/L dexasmison. The cells of the control group were also cultured in the same kind of medium but without any cytokines serving as a control. The expression of hepatic transcriptional factors such as GATA4 and HNF1 were checked by RT-PCR. At the end of the induction, hepatocyte markers were analysed by flow cytometry, and cytokeratin expressions were examined using cyto-immunofluorescence methods.</p><p><b>RESULTS</b>AMSCs grew like fibroblasts and were passaged easily. Most of the third passaged AMSCs were positive against anti-CD29, anti-CD44 antibodies, but negative for the anti-CD34 and anti-CD45 ones. The hepatic transcriptional factor was expressed gradually to higher levels during the induction time. AFP and Alb positive cells were 30.0% and 17.8% of the total cultured cells, and the rate of cells positive to the two markers was 6.9%. The inducted cells were positive for CK18 and CK19 antibodies at the end of the induction. The cells in the control group were negative when checked by these methods.</p><p><b>CONCLUSIONS</b>AMSCs could be directed to differentiate into hepatocytes in vitro by a cytokine cocktail with a low concentration FBS culture system.</p>
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Base de datos:
WPRIM
Asunto principal:
Diferenciación Celular
/
Células Cultivadas
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Adipocitos
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Hepatocitos
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Biología Celular
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Transdiferenciación Celular
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Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua ganzangbing zazhi
Año:
2007
Tipo del documento:
Article