Polymeric nanoparticles with therapeutic gene for gene therapy: I. Preparation and in vivo gene transfer study / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 438-442, 2005.
Article
en Zh
| WPRIM
| ID: wpr-354279
Biblioteca responsable:
WPRO
ABSTRACT
VEGF nanoparticle (VEGF-NP) was prepared by a multi-emulsification technique using a biodegradable poly-dl-lactic-co-glycolic (PLGA) as matrix material. The nanoparticles were characterized for size, VEGF loading capacity, and in vitro release. VEGF-NP and naked VEGF plasmid were intramuscularly injected into the ischemia site of the rabbit chronic hindlimb ischemia model and the efficiency of VEGF-NP as gene delivery carrier for gene therapy in animal model was evaluated. Gene therapuetic effect was assessed evaluated by RT-PCR, immunohistochemistry and angiography assay. The average size of VEGF-NP was around 300 nm. The encapsulation efficiency of VEGF was above 96%. Loading amount of VEGF in the nanoparticles was about 4%. In vitro, nanoparticles maintained sustained-release of VEGF for two weeks. Two weeks post gene injection the capillary density in VEGF-NP group (81.22 per mm2) was significantly higher than that in control group (29.54 mm2). RT-PCR results showed greatly higher VEGF expression in VEGF-NP group (31.79au * mm) than that in naked VEGF group (9.15 au * mm). As a carrier system for gene therapy in animal model, VEGF-NP is much better than naked DNA plasmid. The results demonstrate great possibility of using NP carrier in human gene therapy.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Ácido Poliglicólico
/
Terapia Genética
/
Química
/
Técnicas de Transferencia de Gen
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Ácido Láctico
/
Factor A de Crecimiento Endotelial Vascular
/
Modelos Animales de Enfermedad
/
Nanopartículas
/
Vectores Genéticos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2005
Tipo del documento:
Article