Application of STR genetic marker system in the detection of hemophilia A carriers in Guangxi, China / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 951-955, 2012.
Article
en Zh
| WPRIM
| ID: wpr-353826
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a fast and simple genetic diagnosis technique based on a reliable, short tandem repeat (STR) genetic marker system for the detection of hemophilia A carriers in Guangxi, China.</p><p><b>METHODS</b>Fluorescent PCR and capillary electrophoresis were used for allele genotyping at three intragenic/extragenic STR loci (F8Int13, DXS1073, and DXS9901) of FVIII gene in the members of 10 hemophilia A families in Guangxi, so as to evaluate the diagnostic efficiency of the STR genetic marker system for detection of hemophilia A carriers. Then the STR genetic marker system was used to detect hemophilia A carriers among examinees.</p><p><b>RESULTS</b>In the 10 hemophilia A families, 11 confirmed female carriers had the same allele fragment lengths at the three STR loci (F8Int13, DXS1073, and DXS9901) as the probands. Of the 8 females examined, 5 had allele fragments at the three STR loci (F8Int13, DXS1073, and DXS9901) which were identical to those of the probands, and thus they were diagnosed as hemophilia A carriers.</p><p><b>CONCLUSIONS</b>Genetic analysis at the three STR loci (F8Int13, DXS1073, and DXS9901) can be used to detect hemophilia A carriers rapidly and provide reliable basis for prenatal diagnosis of hemophilia A.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
China
/
Repeticiones de Microsatélite
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Diagnóstico
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Genética
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Genotipo
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Hemofilia A
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Tamización de Portadores Genéticos
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Child
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Child, preschool
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Female
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Humans
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Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2012
Tipo del documento:
Article