Analysis on the metabolites of mesaconitine in the rat urine by liquid chromatography and electrospray ionization mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
; (12): 1043-1047, 2010.
Article
en Zh
| WPRIM
| ID: wpr-353387
Biblioteca responsable:
WPRO
ABSTRACT
The mesaconitine and its major metabolites in the rat urine were identified by liquid chromatography and electrospray ionization tandem mass spectrometry. The rat urine was collected for consecutive 24 hours from the rat following intragastric infusion of mesaconitine, subsequently which were enriched and purified using solid phase extraction. The metabolites of mesaconitine in the rat urine were analyzed by the liquid chromatography and electrospray ionization tandem mass spectrometry. It is shown that the parent drug mesaconitine and its metabolites were found in the rat urine, such as hypo-mesaconitine glucuronic acid conjugate, 10-hydroxy-mesaconitine, 1-O-demethyl mesaconitine, deoxy-mesaconitine and hypo-mesaconitine. Among the five of metabolites, the hypo-mesaconitine glucuronic acid conjugate (m/z 766) was first discovered as the aconitine in rats phase II metabolites, which revealed a new way of mesaconitine metabolism in rats.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Orina
/
Aconitina
/
Estructura Molecular
/
Química
/
Cromatografía Líquida de Alta Presión
/
Ratas Sprague-Dawley
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Espectrometría de Masa por Ionización de Electrospray
/
Aconitum
/
Extracción en Fase Sólida
Límite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2010
Tipo del documento:
Article