Study on post-translational processing and active forms of the novel metastasis-associated protein SNC19 / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 38-42, 2005.
Article
en Zh
| WPRIM
| ID: wpr-353252
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between SNC19 protein and cancer metastasis.</p><p><b>METHODS</b>Expression of SNC19 protein in cancer cell lines and tissues was examined by Western blot analysis using anti-SNC19 monoclonal antibody. In addition, Psectag2A-SNC19(ORF) eukaryotic expression vector was constructed and transfected into BCAP37 cells. After the target protein was expressed and purified, processing forms of SNC19 protein were further identified using anti-His mAb and each form was assayed for its gelatinase activity.</p><p><b>RESULTS</b>Different expression and post-translational processing of the SNC19 proteins in the cancer cell lines and intestinal tissues were detected.BCAP37 cells transfected full-length SNC19 (ORF) generated two different sized proteins in cell lysates, 120 and 75 kD; 75 kD was detected to have proteolytic activity by gelatin zymography.</p><p><b>CONCLUSION</b>SNC19 protein presents different expression and post-translational processing in the cancer cells and tissues, of which 75 kD was identified to have gelatinase activity.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Neoplasias de la Mama
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Serina Endopeptidasas
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Transfección
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Química
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Procesamiento Proteico-Postraduccional
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Neoplasias del Colon
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Gelatinasas
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ADN Complementario
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Línea Celular Tumoral
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2005
Tipo del documento:
Article