Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1699-1703, 2012.
Article
en Zh
| WPRIM
| ID: wpr-352352
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Qufengtongluo Recipe (QFTLR) on the expressions of cell cycle regulatory proteins in rat mesangial cells (MCs) in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.</p><p><b>METHODS</b>Using the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry.</p><p><b>RESULTS</b>Compared with the normal control cells, the cells challenged with lipopolysaccharide (LPS) showed significantly enhanced expressions of cyclin D1, CDK2, and P21 (P<0.01) and obviously lowered protein expression of P27 (P<0.01). Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21 and obvious increase of P27 expression (P<0.05 or P<0.01), but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21 and P27 protein expressions (P<0.05 or P<0.01).</p><p><b>CONCLUSION</b>QFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1, CDK2, and P21 and up-regulating the expression of P27 protein.</p>
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Base de datos:
WPRIM
Asunto principal:
Farmacología
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Medicamentos Herbarios Chinos
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Línea Celular
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Regulación de la Expresión Génica
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Ratas Sprague-Dawley
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Ciclina D1
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Biología Celular
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Células Mesangiales
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Quinasa 2 Dependiente de la Ciclina
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Inhibidor p21 de las Quinasas Dependientes de la Ciclina
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Article