Construction of Runx1 transgenic mice / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 584-587, 2013.
Article
en Zh
| WPRIM
| ID: wpr-352205
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3. 1 Flag Runx1 FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection technology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runx1 transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Recombinación Genética
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Ratones Transgénicos
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Datos de Secuencia Molecular
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Secuencia de Bases
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Subunidad alfa 2 del Factor de Unión al Sitio Principal
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Vectores Genéticos
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Genética
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Ratones Endogámicos C57BL
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Microinyecciones
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2013
Tipo del documento:
Article