Comparison of 2,3-butanediol production by several strains and optimization of the fermentation medium / 生物工程学报
Chinese Journal of Biotechnology
; (12): 489-492, 2011.
Article
en Zh
| WPRIM
| ID: wpr-351508
Biblioteca responsable:
WPRO
ABSTRACT
Five Klebsiella pneumonia strains (including two strains whose genes for lactic acid were knocked out) were used to produce 2,3-butanediol, in which K. pneumonia HR521 LDH (gene for lactic acid was knocked out) was the best for the production, and then the fermentation medium was optimized by orthogonal design. The optimum compositions were as follows: glucose 90 g/L, (NH4)2HPO4 3 g/L, CLSP 6 g/L, sodium acetate 5 g/L, KCl 0.4 g/L, MgSO4 0.1 g/L, FeSO4 x 7H2O 0.02 g/L, MnSO4 0.01 g/L. Under the above conditions, final concentration of acetone and 2,3-butanediol could reach 37.46 g/L, 10 g/L higher than that under the initial conditions, the yield was 90.53% of the theory, and the productivity was 1.5 g/(L-h), and no lactic acid was detected, which could be benefit for the downstream processing and industrial application.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Butileno Glicoles
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Química
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Clasificación
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Medios de Cultivo
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Técnicas de Inactivación de Genes
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Fermentación
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Genética
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Glucosa
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Klebsiella pneumoniae
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Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article