Expression and function of autophagy after ischemia/reperfusion in rats hippocampus neuron / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 187-191, 2011.
Article
en Zh
| WPRIM
| ID: wpr-351203
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of autophagy after ischemia/reperfusion and its possible function in rats hippocampus neurons.</p><p><b>METHODS</b>After 2 hours oxygen-glucose deprivation and different periods time of reperfusion (OGD/R) treatment in primary hippocampal neurons, neuron viability was evaluated by MTT assay, specific structure of autophagosome and specific protein of autophagy microtubule-associated protein 1 light chain 3 B (LC3B) were detected by transmission electron microscope and immunofluorescence respectively. The inhibitor of autophagy 3-Methyladenine (3-MA) was also used to exam the viability of neurons.</p><p><b>RESULTS</b>Treatment by OGD/R markedly reduced neuronal viability. Compared to the control group, autophagy existed in different time periods after OGD/R shown both in transmission electron microscope and immunofluorescence. Application of 3-MA significantly reduced neuronal viability.</p><p><b>CONCLUSION</b>Oxygen-glucose deprivation can activate autophagy in rat hippocampus neurons, which may resist the injury during ischemia/reperfusion.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Fisiología
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Autofagia
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Daño por Reperfusión
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Hipoxia de la Célula
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Isquemia Encefálica
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Medio de Cultivo Libre de Suero
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Ratas Sprague-Dawley
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Biología Celular
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Cultivo Primario de Células
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2011
Tipo del documento:
Article