Detection of microvesicle miRNA expression in ALL subtypes and analysis of their functional roles / 华中科技大学学报（医学）（英德文版）
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 640-645, 2014.
Article
en En
| WPRIM
| ID: wpr-351025
Biblioteca responsable:
WPRO
ABSTRACT
Microvesicles (MVs) are the heterogeneous mixtures of vesicles. MVs released by leukemia cells constitute an important part of the leukemia microenvironment. MVs might act as important reservoirs of microRNAs (miRNAs). It is worth evaluating whether MVs possess some unique miRNA contents that are valuable in understanding the pathogenesis. In this study, we investigated the miRNA expression patterns of Nalm-6-derived MVs, Jurkat-derived MVs and normal cell-derived MVs using miRNA microarrays. The potential target genes regulated by differentially expressed miRNAs were also predicted and analyzed. Results demonstrated that 182 miRNAs and 166 miRNAs were differentially expressed in Nalm-6-MVs and Jurkat-MVs, respectively. Many oncogenes, tumor suppressors and signal pathway genes were targeted by these aberrantly expressed miRNAs, which might contribute to the development of B-ALL or T-ALL. Our findings expanded the potential diagnostic markers of ALL and provided useful information for ALL pathogenesis.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Leucemia-Linfoma Linfoblástico de Células Precursoras B
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Regulación Leucémica de la Expresión Génica
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Células Jurkat
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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MicroARNs
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Leucemia-Linfoma Linfoblástico de Células T Precursoras
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Cuerpos Multivesiculares
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2014
Tipo del documento:
Article