Differentiation character of adult mesenchymal stem cells and transfection of MSCs with lentiviral vectors / 华中科技大学学报（医学）（英德文版）
J. huazhong univ. sci. tech. med. sci
; (6): 687-693, 2010.
Article
en En
| WPRIM
| ID: wpr-349760
Biblioteca responsable:
WPRO
ABSTRACT
This study examined the differentiation character and pluripotency of mesenchymal stem cells (MSCs) under different conditions. Adult MSCs were initially isolated from the bone marrow of rats, cultured in vitro and identified by flow cytometry. After MSCs were transferred to osteogenic and adipogenic medium respectively, the morphological characterization of induced cells was observed. The expression of marker genes was detected by RT-PCR analysis. Then MSCs were transfected with lentiviral vectors pGC-FU-Sox9-EGFP. Enhanced green fluorescence protein (EGFP) expression and transfection efficiency were determined by fluorescence microscopy. The results demonstrated that EGFP caused no effect on the multilineage potential of adult MSCs. Sox9 gene expression of high level was maintained stable in the transfected MSCs and induced MSCs to differentiate into chondrocytes. Aggracan was positive in chondrogenic lineages and the expression of aggracan and type collagen II was significantly increased during MSCs chondrogenic differentiation. It was concluded that Sox9 gene-modified adult MSCs may be promising candidate cells for further studies on tissue engineering. EGFP facilitates the research on MSCs physiological behavior and application in tissue engineering during differentiation both in vitro and in vivo.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Células de la Médula Ósea
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Transfección
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Diferenciación Celular
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Células Cultivadas
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Lentivirus
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Condrocitos
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Biología Celular
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Colágeno Tipo II
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Ingeniería de Tejidos
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Proteínas Fluorescentes Verdes
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J. huazhong univ. sci. tech. med. sci
Año:
2010
Tipo del documento:
Article