Polymorphism of fifteen short tandem repeat loci in Tibetan of Changdu area / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 469-473, 2009.
Article
en Zh
| WPRIM
| ID: wpr-349051
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic polymorphism of 15 autosomal short tandem repeat (STR) loci, i.e. D5S818, FGA, D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX and D18S51, in Tibetan population of Changdu area, and to analyze the genetic relationship between this population and other Asian populations.</p><p><b>METHODS</b>The 15 loci were amplified simultaneously using a multiplex PCR typing system. Amplified STR fragments were analyzed with an ABI PRISM 3100 Avant DNA Analyzer. Arlequin software version 3.1 was used to obtain allele frequencies and polymorphism parameters.</p><p><b>RESULTS</b>There were 135 STR alleles in the 15 STRs of Changdu Tibetan, with allele frequencies ranging from 0.0065 to 0.5455. The average heterozygosity was 0.7340, the discrimination power was higher than 0.8 except for TPOX and TH01. The accumulative discrimination power was more than 0.9999998, and the accumulative non-parental exclusion probability was 0.99999997.</p><p><b>CONCLUSION</b>The 15 STR loci of Changdu Tibetan possess characteristics of high genetic diversity. They can be employed in minority genetics investigation, and individual and paternity test in forensic medicine.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Linaje
/
Polimorfismo Genético
/
Tibet
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Repeticiones de Microsatélite
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Pueblo Asiatico
/
Etnología
/
Genética
Límite:
Female
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Humans
/
Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Año:
2009
Tipo del documento:
Article