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Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2 / 药学学报
Yao Xue Xue Bao ; (12): 438-442, 2011.
Article en Zh | WPRIM | ID: wpr-348937
Biblioteca responsable: WPRO
ABSTRACT
The inhibitory ratio (1%) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and 1% was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC50) was about 2.26 mg x mL(-1). There was a good relationship between the logarithm concentrations of artificial musk and 1% when the concentrations of artificial musk ranged from 0.31-20.0 mg x mL(-1). The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.
Asunto(s)
Texto completo: 1 Base de datos: WPRIM Asunto principal: Farmacología / Ácidos Grasos Monoinsaturados / Técnicas para Inmunoenzimas / Concentración 50 Inhibidora / Relación Estructura-Actividad Cuantitativa / Ciclooxigenasa 2 / Inhibidores de la Ciclooxigenasa 2 / Metabolismo / Métodos / Antiinflamatorios Idioma: Zh Revista: Yao Xue Xue Bao Año: 2011 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Asunto principal: Farmacología / Ácidos Grasos Monoinsaturados / Técnicas para Inmunoenzimas / Concentración 50 Inhibidora / Relación Estructura-Actividad Cuantitativa / Ciclooxigenasa 2 / Inhibidores de la Ciclooxigenasa 2 / Metabolismo / Métodos / Antiinflamatorios Idioma: Zh Revista: Yao Xue Xue Bao Año: 2011 Tipo del documento: Article