Effects of glycine on apoptosis in murine cardiomyocyte suffering from ischemia and hypoxia / 中华烧伤杂志
Chinese Journal of Burns
; (6): 167-170, 2008.
Article
en Zh
| WPRIM
| ID: wpr-347623
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of glycine on apoptosis in murine cardiomyocyte suffering from ischemia and hypoxia.</p><p><b>METHODS</b>The primary passage of cultured cardiomyocytes from neonatal rats were subjected to ischemia and hypoxia, and the cells were divided into IH (without other treatment), and G (with treatment of 5 mmol/L glycine) groups. Normal murine cardiomyocytes served as control (C group). Cardiomyocytes were cultured for 6 hours in vitro. Apoptosis, mitochondrial membrane potential and its distribution, the condition of mitochondria permeability transition pore (mPTP) were observed with expression of fluorescence intensity. The activity of caspase-3 was observed by Laser Scanning staining.</p><p><b>RESULTS</b>(1) Apoptosis: the fluorescence intensity in IH group was obviously higher than that in G and C groups (P < 0.01). (2) Mitochondrial membrane potential: the fluorescence intensity in IH group was 32 +/- 7, which was obviously lower than that in G and C groups (52 +/- 4, 73 +/- 4, respectively, P < 0.01). (3) The condition of mPTP: the intensity in IH group was 27 +/- 4, which was obviously lower than that in G and C groups (62 +/- 8, 90 +/- 7, respectively, P < 0.01). (4) The activity of caspase-3: the activity of caspase-3 in IH group was obviously higher than that in G and C groups (P < 0.01).</p><p><b>CONCLUSION</b>Glycine can inhibit apoptosis in cardiomyocytes subjected to ischemia and hypoxia,and the effect may be attributable to changes in mitochondrial membrane potential, lessening opening of mPTP, alleviation of calcium overload , and decrease in activity of caspase-3.</p>
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Base de datos:
WPRIM
Asunto principal:
Farmacología
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Hipoxia de la Célula
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Células Cultivadas
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Ratas Sprague-Dawley
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Apoptosis
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Biología Celular
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Miocitos Cardíacos
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Caspasa 3
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Glicina
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Isquemia
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Burns
Año:
2008
Tipo del documento:
Article