Effect of human serum albumin on cell attachment of human gingival epithelial cells / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 197-199, 2002.
Article
en En
| WPRIM
| ID: wpr-347396
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of human serum albumin (HSA) on cell attachment of human gingival epithelial cells (HGE).</p><p><b>METHODS</b>HGE were primary cultured with keratinocyte serum-free medium (KSFM) and dispase. The cultured cells were immunohistochemically stained by monoclonal anti-pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed.</p><p><b>RESULTS</b>The results showed significant decrease in cell numbers within 8 hours after HGE were inoculated, in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference.</p><p><b>CONCLUSIONS</b>HSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Poliestirenos
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Albúmina Sérica
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Adhesión Celular
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Recuento de Células
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División Celular
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Células Cultivadas
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Biología Celular
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Células Epiteliales
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Encía
Límite:
Humans
Idioma:
En
Revista:
Chinese Journal of Stomatology
Año:
2002
Tipo del documento:
Article