Regulation mechanism of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes in vitro and in vivo / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3637-3640, 2012.
Article
en Zh
| WPRIM
| ID: wpr-346892
Biblioteca responsable:
WPRO
ABSTRACT
To study the effects of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro, and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST, NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase II detoxification enzyme activity and protein expression.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Triterpenos
/
Medicamentos Herbarios Chinos
/
Química
/
NAD(P)H Deshidrogenasa (Quinona)
/
Prunella
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Línea Celular Tumoral
/
Factor 2 Relacionado con NF-E2
/
Fase II de la Desintoxicación Metabólica
/
Genética
/
Glutatión
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Article