Comparison between icariin and genistein in osteogenic activity of marrow stromal cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1783-1788, 2013.
Article
en Zh
| WPRIM
| ID: wpr-346499
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC).</p><p><b>METHOD</b>Rat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h.</p><p><b>RESULT</b>Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) could increase the activity of ALP and the content of Ca, regulate OXS, BMP-2, Runx-2 and Collagen-I mRNA expression.</p><p><b>CONCLUSION</b>Icariin showed a stronger effect in improving the osteogenic differentiation of rat bone marrow stromal cells than genistein.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Osteogénesis
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Farmacología
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Flavonoides
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Expresión Génica
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Diferenciación Celular
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Células Cultivadas
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Factor de Crecimiento Transformador beta
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Ratas Sprague-Dawley
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Genisteína
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Biología Celular
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2013
Tipo del documento:
Article