A method for PCR product cloning based on exonuclease III / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1266-1273, 2014.
Article
en Zh
| WPRIM
| ID: wpr-345598
Biblioteca responsable:
WPRO
ABSTRACT
Gene cloning is one of the most important and widely used technologies in molecular biology research. Generally, DNA fragment is cut with restriction enzyme, and then the product is ligated to a linearized vector with complementary sticky end or blunt end by DNA-ligase. This traditional DNA cloning method requires compatible enzyme recognition sites existing in both PCR fragment and targeted vector. Several ligase-free methods have been established to avoid the using of restriction enzyme. However, those methods are time-consuming, labor-intensive and expensive. To overcome these shortcomings, we developed an Exonuclease III based DNA cloning method that takes only 30 minutes with high cloning efficiency and significant economic advantage. Therefore, this method is suitable for large-scale gene cloning.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
ADN
/
Enzimas de Restricción del ADN
/
Química
/
Reacción en Cadena de la Polimerasa
/
Clonación Molecular
/
Exodesoxirribonucleasas
/
Vectores Genéticos
/
Métodos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Article