Expression of human ERMAP gene in different cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 819-822, 2005.
Article
en Zh
| WPRIM
| ID: wpr-343879
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the pattern of human ERMAP gene expression in different cell lines, 15 cell lines derived from hematopoietic tumor, somatic tumor and normal tissue were chosen and were cultured; cells were harvested after culture for 12, 24, 36, 48, 60 and 72 hours; the expression of the human ERMAP was detected by using fluorescent quantitative PCR. The results showed that human ERMAP gene expression was positive in K562 cell line at interval of 12 and 24 hours, and the expression levels were (5.092 +/- 2.331) x 10(6) cps/microl RNA, (5.328 +/- 3.916) x 10(6) cps/microl RNA, respectively; ERMAP gene expression was also positive in ECV304 cell line at interval of 24 hours, and the expression level was (0.84 +/- 0.12) x 10(6) cps/microl RNA; and its expression was negative in other 13 cell lines. It is concluded that human ERMAP gene expression in ECV304 cell line was found first, and its expression in K562 cell line was further confirmed.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Antígenos de Grupos Sanguíneos
/
ARN Mensajero
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Regulación Neoplásica de la Expresión Génica
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Línea Celular
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Células HL-60
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Células Jurkat
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Neoplasias Hematológicas
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Células K562
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Perfilación de la Expresión Génica
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Article