Detection of APC gene promoter methylation in hematological malignant cell lines by nested-methylation specific polymerase chain reaction / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 957-960, 2009.
Article
en Zh
| WPRIM
| ID: wpr-343372
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to investigate the efficiency of nested methylation specific polymerase chain reaction (nMS-PCR) for detecting the APC gene promoter methylation and to clarify the roles of methylation in genesis and development of hematologic malignancies, as well as to screen the hematologic malignant cell lines with hypermethylation of APC gene promoter to use as an ideal cell model for exploring the relationship between gen methylation and gene expression. The genome DNA of 10 cell lines modified with bisulfide was amplified and the methylation status of APC gene promoter was detected by using nMS-PCR. The results showed that the methylation of APC gene promoter was detected in Jurkat cells, while could not be detected in CA46, U266, Molt4, K562, HL-60, CEM, AKR, U937 and Raji cell lines. In conclusion, APC gene methylation in hematological malignant cell lines can be accurately detected by nMS-PCP method, which is simple method for detecting methylation status of various hematological malignant cell lines.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Regiones Promotoras Genéticas
/
Genes APC
/
Células HL-60
/
Metilación de ADN
/
Células K562
/
Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Article