Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1234-1237, 2009.
Article
en Zh
| WPRIM
| ID: wpr-343312
Biblioteca responsable:
WPRO
ABSTRACT
This study was purposed to investigate the practicality of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for detection of single nucleotide polymorphisms (SNP) on jak2 gene in multiple myeloma (MM) cells. The DNA fragment containing 2 SNPs of jak2 (C428T and C643T) was amplified using PCR and was purified. The purified product was used as the template for primer extension (PEX). The small products of allele-specific reaction were purified, the SNPs on jak2 gene of 5 patients with MM and 5 healthy persons were detected by MALDI-TOF MS. The results showed that the distribution of genotype C428T and C643T was not different between MM patients and healthy persons, both of which are homozygous T/T. In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Estudios de Casos y Controles
/
Cartilla de ADN
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Polimorfismo de Nucleótido Simple
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Janus Quinasa 2
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Genética
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Genotipo
/
Métodos
/
Mieloma Múltiple
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Article