Investigation of uninterpretative HLA typing in 311 umbilical cord blood samples / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1261-1264, 2009.
Article
en Zh
| WPRIM
| ID: wpr-343306
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to investigate the factors which affect HLA typing in 311 umbilical cord blood (UCB) samples. The HLA low resolution typing of UCB samples with misinterpreted HLA types from 311 UCB samples analyzed by PCR-SSO and PCR-SSP was performed. 7 samples difficult to determine their HLA genotype were sequenced directly and the reason leading to misinterpret HLA typing was analyzed. The results indicated that 99.4% of misinterpreted samples resulted from the restriction of HLA typing method itself and 0.6% of misinterpreted samples were suspected to be contaminated with maternal blood in UCB. It is concluded that HLA typing is mainly affected by the shortcomings of oligonucleotide probe design for PCR-SSO and lack of allele specific primers of PCR-SSP.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Prueba de Histocompatibilidad
/
Secuencia de Bases
/
Sondas de Oligonucleótidos
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Alelos
/
Alergia e Inmunología
/
Sangre Fetal
/
Genética
/
Genotipo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Article