Study on the correlation between positive rates of SARS RNA in clinical confirmed SARS patients and the appearance of RNA in relation to the development of the disease / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 120-123, 2004.
Article
en Zh
| WPRIM
| ID: wpr-342373
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To study the correlation between positive rates of RNA in clinical confirmed severe acute respiratory syndrome (SARS) patients and its appearance in relation to the development of the disease in order to provide scientific basis for early diagnosis, effective prevention and treatment of the disease.</p><p><b>METHODS</b>One-step reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the SARS RNA in the clinical specimens from different courses of the disease. The representative amplicons were then sequenced. Chi-square for trend test was performed to study the correlation between positive rates of RT-PCR and at different periods after the onset of the disease.</p><p><b>RESULTS</b>The fragments amplified from the sputum specimens of SARS patients were shown to share 100% homology with the published SARS-associated coronavirus. Of the different clinical specimens, positive rate in the stools appeared to be the highest (21.55%). Chi-square for trend test revealed that the positive rates of stools and sputa of SARS patients decreased with the development of the disease (chi(2) for trend = 12.55 and 16.408, P = 0.0004 and P = 0.000 05 respectively).</p><p><b>CONCLUSION</b>One-step RT-PCR proved to be an effective method for the detection of SARS-associated coronavirus from clinical specimens. Data as indicated that the positive rates of SARS coronavirus were decreasing in SARS patients along with the disease progression.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Virología
/
ARN Viral
/
Distribución de Chi-Cuadrado
/
China
/
Progresión de la Enfermedad
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Síndrome Respiratorio Agudo Grave
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Heces
Tipo de estudio:
Screening_studies
Límite:
Adolescent
/
Adult
/
Aged
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Female
/
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Año:
2004
Tipo del documento:
Article